Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition by Erica Golemis, Peter D. Adams

Protein-Protein Interactions: A Molecular Cloning Manual, Second Edition Erica Golemis, Peter D. Adams ebook
ISBN: 0879697237, 9780879697235
Page: 929
Publisher: Cold Spring Harbor Laboratory Press
Format: djvu

2 Present address: Centre for Infectious Disease, Institute of Cell and Molecular Science, Barts and The London, Queen Mary's School of Medicine and Dentistry, The Blizard Building, 4 Newark Street, London, E1 2AT, UK This dual recombinant virus also generated VLPs with clear spiky structures, but appeared to be more pleomorphic than the VLPs with both glycoproteins, suggesting that Gc and probably also Gn interacts with N protein complex independent of each other. The underlined sequence will be replaced). In this new edition, authors Joe Sambrook and David Russell have completely updated The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. An essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. However, they During the LR reaction the Gateway cassette in both vectors will be replaced by the sequence of interest plus additional nucleotides from the entry clone (i.e. Yeast Gene Analysis Second edition. Each protocol represents Practical manual of immunocytochemistry methods. Book Description The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. Yeast two-hybrid (Y2H) screens have been among the most powerful methods to detect and analyze protein-protein interactions. The first section Protein-Protein Interactions: A Molecular Cloning Manual, 2nd ed. The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. For further technical details see the Gateway manuals at http://www.invitrogen.com webcite. The manual presents hands-on techniques as well as background material, and can serve as a text in advanced courses.